Development of x-ray laminography under an x-ray microscopic condition

被引：21

作者：

Hoshino, Masato ^{[1
]}

Uesugi, Kentaro ^{[1
]}

Takeuchi, Akihisa ^{[1
]}

Suzuki, Yoshio ^{[1
]}

Yagi, Naoto ^{[1
]}

机构：

[1] Japan Synchrotron Radiat Res Inst JASRI SPring 8, Sayo, Hyogo 6795198, Japan

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2011年 / 82卷 / 07期

关键词：

biomedical imaging; biomembranes; cellular biophysics; X-ray microscopy; zone plates; HIGH-SPATIAL-RESOLUTION; PHASE-CONTRAST; COMPUTED-TOMOGRAPHY; SPECIMENS; RADIATION;

D O I：

10.1063/1.3609865

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

An x-ray laminography system under an x-ray microscopic condition was developed to obtain a three-dimensional structure of laterally-extended planar objects which were difficult to observe by x-ray tomography. An x-ray laminography technique was introduced to an x-ray transmission microscope with zone plate optics. Three prototype sample holders were evaluated for x-ray imaging laminography. Layered copper grid sheets were imaged as a laminated sample. Diatomite powder on a silicon nitride membrane was measured to confirm the applicability of this method to non-planar micro-specimens placed on the membrane. The three-dimensional information of diatom shells on the membrane was obtained at a spatial resolution of sub-micron. Images of biological cells on the membrane were also obtained by using a Zernike phase contrast technique. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3609865]

引用

页数：6

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