Hybrid artificial liver using hepatocyte organoid culture

被引:76
作者
Funatsu, K
Ijima, H
Nakazawa, K
Yamashita, Y
Shimada, M
Sugimachi, K
机构
[1] Kyushu Univ, Fac Engn, Dept Chem Engn, Higashi Ku, Fukuoka 8128581, Japan
[2] Kyushu Univ, Fac Med Sci, Dept Surg & Sci, Higashi Ku, Fukuoka 8128581, Japan
关键词
hybrid artificial liver support system; polyurethane foam; spheroid; hollow fiber; hepatocyte organoid; preclinical experiment;
D O I
10.1046/j.1525-1594.2001.025003194.x
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We developed 2 types of hybrid artificial liver modules using hepatocyte organoid culture. One was a polyurethane foam (PUF)/hepatocyte spheroid packed-bed module. Hepatocytes spontaneously formed spheroids in the PUF pores, and they maintained liver-specific functions well for at least 2 weeks in vitro. As a preclinical experiment, a hybrid artificial liver with 200 g porcine hepatocytes was applied to a pig (25 kg) with liver failure and showed that the hybrid artificial liver was effective in support of Liver functions and stabilization of general conditions. We established a new technique of hepatocyte organoid formation using centrifugal force. A hepatocyte organoid formed by centrifugation in hollow fibers maintained functions for more than 4 months in vitro. We developed a new sinusoid-like structure module having hollow fibers arranged by spacers in a micro-regular arrangement. Inoculated hepatocytes in the extra-fiber space of the module formed the organoid by centrifugation, and they maintained the functions for at least 1 month in vitro. The results indicated that this module seems to be promising as a hybrid artificial liver.
引用
收藏
页码:194 / 200
页数:7
相关论文
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