Graphene-based biosensors for detection of bacteria and their metabolic activities

被引:289
作者
Huang, Yinxi [1 ]
Dong, Xiaochen [2 ,3 ]
Liu, Yuxin [1 ]
Li, Lain-Jong [4 ]
Chen, Peng [1 ]
机构
[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, Singapore 637457, Singapore
[2] Nanjing Univ Posts & Telecommun, Key Lab Organ Elect & Informat Displays, Nanjing 210046, Peoples R China
[3] Nanjing Univ Posts & Telecommun, Inst Adv Mat, Nanjing 210046, Peoples R China
[4] Acad Sinica, Res Ctr Appl Sci, Taipei 11529, Taiwan
关键词
FIELD-EFFECT TRANSISTORS; CARBON-NANOTUBE-NETWORK; CELLULAR BIOELECTRICITY; INHIBITION; NANOSHEETS; NANOWIRES; SECRETION; DEVICES; ARRAYS; SENSOR;
D O I
10.1039/c1jm11436k
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Graphene, which is a recently discovered single-atom-thick planar sheet of carbon atoms perfectly arranged in a honeycomb lattice, has great potential in biosensing owing to its extraordinary electrical, physical, and optical properties. In this work, we demonstrate a graphene based biosensor to electrically detect E. coli bacteria with high sensitivity and specificity. The large-sized graphene film was grown by chemical vapor deposition and functionalized with anti-E. coli antibodies and passivation layer. Significant conductance increase of the graphene device was observed after exposure to E. coli bacteria at a concentration as low as 10 cfu/mL, while no significant response was triggered by high concentration of the another bacterial strain. In addition, this biosensor was employed to detect the glucose induced metabolic activities of the bound E. coli bacteria in real time. This simple, fast, sensitive, and label-free nanoelectronic biosensor, in principle, could serve as a high throughput platform for detection of any pathogenic bacteria, and for functional studies or screening of antibacterial drugs.
引用
收藏
页码:12358 / 12362
页数:5
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