Immobilized-metal affinity sorbents based on hydrophilic methacrylate polymers and their interaction with immunoglobulins

被引：15

作者：

Mézárosová, K ^{[1
]}

Tishchenko, G ^{[1
]}

Bouchal, K ^{[1
]}

Bleha, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Macromol Chem, Prague 16206, Czech Republic

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2003年 / 56卷 / 01期

关键词：

poly[2-(2-hydroxyethoxy)ethyl methacrylate-co-ethylene dimethacrylate; ethylenediaminetriacetic acid; quinolin-8-ol; N-(2-pyridylmethyl)glycine; IMA sorbents;

D O I：

10.1016/S1381-5148(03)00029-4

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Hydrophilic homogeneous poly [2-(2-hydroxyethoxy)ethyl methacrylate-co-ethylene dimethacrylate] poly(DEGMA-co-EDMA) beads have been prepared as precursors for introducing the chelating groupings of ethylenediaminetriacetic acid, quinolin-8-ol, or N-(2-pyridylmethyl)glycine. The effect of the chelating ligand structure on coordination with Ni2+ ions and that of the metal complexes on binding the model protein (horseradish-peroxidase-specific immunoglobulin IgG(1)) was analyzed. It was found that the protein binding affinity and selectivity was the highest for IMA sorbents with quinolin-8-ol groups. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：27 / 35

页数：9

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