The construction of an amperometric immunosensor for the thyroid hormone (+)-3,3′,5-triiodo-L-thyronine (L-T3)

被引：11

作者：

Aboul-Enein, HY

Stefan, RI

Radu, GL

Baiulescu, GE

机构：

[1] King Faisal Specialist Hosp & Res Ctr, Dept Biol & Med Res MBC 03, Bioanalyt & Drug Dev Lab, Riyadh 11211, Saudi Arabia

[2] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest 70346, Romania

[3] Natl Inst Biol Sci, Dept Analyt Biochem, Bucharest 77748, Romania

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 03期

关键词：

amperometric immunosensor; graphite paste; thyroid hormone; (+)-3,3 ',5-triiodo-L-thyronine; mouse monoclonal anti-(+)-3,3 ',5-triiodo-L-thyronine;

D O I：

10.1080/00032719908542832

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel amperometric immunosensor based on graphite paste (graphite powder and paraffin oil) has been constructed. The graphite paste is impregnated with mouse monoclonal anti-(+)-3,3',5-triiodo-L-thyronine (anti-T-3). The immunosensor can be reliably used for L-T-3 assay in thyroid and in drugs, using chronoamperometric technique, at ppb-ppt concentration levels. The potential used for L-T3 assay was 650 mV vs Ag/AgCl. The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new assay.

引用

页码：447 / 455

页数：9

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