Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infection

被引:84
作者
Lesser, CF
Miller, SI
机构
[1] Univ Washington, Dept Med, Seattle, WA 98195 USA
[2] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
关键词
actin cytoskeleton; cell cycle; Salmonella; type III secretion; Yersinia;
D O I
10.1093/emboj/20.8.1840
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacterial virulence proteins that are translocated into eukaryotic cells were expressed in Saccharomyces cerevisiae to model human infection. The subcellular Localization patterns of these proteins in yeast paralleled those previously observed during mammalian infection, including localization to the nucleus and plasma membrane, Localization of Salmonella SspA in yeast provided the first evidence that SspA interacts with actin in living cells. In many cases, expression of the bacterial virulence proteins conferred genetically exploitable growth phenotypes. In this way, Yersinia YopE toxicity was demonstrated to be linked to its Rho GTPase activating protein activity. YopE blocked polarization of the yeast cytoskeleton and cell cycle progression, while SspA altered polarity and inhibited depolymerization of the actin cytoskeleton, These activities are consistent with previously proposed or demonstrated effects on higher eukaryotes and provide new insights into the roles of these proteins in pathogenesis: SspA in directing formation of membrane ruffles and YopE in arresting cell division. Thus, study of bacterial virulence proteins in yeast is a powerful system to determine functions of these proteins, probe eukaryotic cellular processes and model mammalian infection.
引用
收藏
页码:1840 / 1849
页数:10
相关论文
共 37 条
  • [21] The cytolethal distending toxin family
    Pickett, CL
    Whitehouse, CA
    [J]. TRENDS IN MICROBIOLOGY, 1999, 7 (07) : 292 - 297
  • [22] PRINGLE JR, 1989, METHOD CELL BIOL, V31, P357
  • [23] Pruyne D, 2000, J CELL SCI, V113, P365
  • [24] THE SMALL GTP-BINDING PROTEIN RHO REGULATES THE ASSEMBLY OF FOCAL ADHESIONS AND ACTIN STRESS FIBERS IN RESPONSE TO GROWTH-FACTORS
    RIDLEY, AJ
    HALL, A
    [J]. CELL, 1992, 70 (03) : 389 - 399
  • [25] INTRACELLULAR TARGETING OF THE YERSINIA YOPE CYTOTOXIN IN MAMMALIAN-CELLS INDUCES ACTIN MICROFILAMENT DISRUPTION
    ROSQVIST, R
    FORSBERG, A
    WOLFWATZ, H
    [J]. INFECTION AND IMMUNITY, 1991, 59 (12) : 4562 - 4569
  • [26] TARGET-CELL CONTACT TRIGGERS EXPRESSION AND POLARIZED TRANSFER OF YERSINIA YOPE CYTOTOXIN INTO MAMMALIAN-CELLS
    ROSQVIST, R
    MAGNUSSON, KE
    WOLFWATZ, H
    [J]. EMBO JOURNAL, 1994, 13 (04) : 964 - 972
  • [27] THE CYTOTOXIC PROTEIN YOPE OF YERSINIA OBSTRUCTS THE PRIMARY HOST DEFENSE
    ROSQVIST, R
    FORSBERG, A
    RIMPILAINEN, M
    BERGMAN, T
    WOLFWATZ, H
    [J]. MOLECULAR MICROBIOLOGY, 1990, 4 (04) : 657 - 667
  • [28] MICROINJECTION OF THE YERSINIA YOPE CYTOTOXIN IN MAMMALIAN-CELLS INDUCES ACTIN MICROFILAMENT DISRUPTION
    ROSQVIST, R
    FORSBERG, A
    WOLFWATZ, H
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (04) : 1131 - 1132
  • [29] Nonspecific modification of cellular immunity by Yersinia enterocolitica
    RuizBravo, A
    JimenezValera, M
    Roman, SM
    [J]. IMMUNOLOGY LETTERS, 1996, 49 (1-2) : 57 - 61
  • [30] SIKORSKI RS, 1989, GENETICS, V122, P19