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Contamination free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications

被引：94
作者：
Dorfman, KD
Chabert, M
Codarbox, JH
Rousseau, G
de Cremoux, P
Viovy, JL
机构：
[1] Inst Curie, Sect Rec, Lab Physicochim Curie, CNRS UMR 168, F-75248 Paris, France
[2] Inst Curie, Sect Rec, Pharmacol Unit, F-75248 Paris, France
来源：
ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 11期
关键词：
D O I：
10.1021/ac050031i
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We present a method for performing polymerase chain reaction (PCR) using isolated droplets flowing in an immiscible fluorinated solvent system. Thanks to an optimized control of interfacial properties, we could achieve in this capillary-based system reproducible amplification factors, without any detectable contamination between neighboring droplets. The system is readily amenable to further miniaturization and automation and serves as the first step toward a clinically viable, high-throughput, quantitative continuous flow PCR apparatus.
引用
收藏
页码：3700 / 3704
页数：5
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