Determination of diclofenac sodium, flufenamic acid, indomethacin and ketoprofen by LC-APCI-MS

A sensitive, selective and accurate high-performance liquid chromatography-mass spectrometry (LC-MS) assay for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs), namely diclofenac sodium (DIC), flufenamic acid (FLU), indomethacin (IND) and ketoprofen (KET), tither individually or in mixtures, was developed. The examined drugs were injected onto Shim-pack GLC-CN column and were eluted with a mobile phase consisting of acetonitrile and 20 mM ammonium acetate solution (5:1 v/v)/pH 7.4 at a flow rate 1 ml min(-1). The mass spectrometer. operated in the single ion monitoring mode, was programmed to admit the negative ions [M-H](-) at m/z 295.9 (DIC), 280.1 (FLU), 355.8 (IND) and 252.9 (KET), respectively. The calibration curves were linear (r greater than or equal to 0.9993) over the concentration range 50-300 ng ml(-1) (FLU, DIG) and 100-500 ng ml(-1) (KET, IND) with detection limits of 0.5-3.0 ng. The mean predicted concentrations for the analytes were in the range - 5.9 and 5.2% of the nominal concentrations. Within-day and between-day precision were in the range of 0.8-9.1% of the R.S.D. Mean recovery percentages of the individual compounds from laboratory-made mixtures and pharmaceutical formulations were (99.5-101.5%) and (100.6-102.2%), respectively. (C) 2001 Elsevier Science B.V. All rights reserved.