Reverse genetics of coronaviruses using vaccinia virus vectors

被引:25
作者
Thiel, V [1 ]
Siddell, SG
机构
[1] Cantonal Hosp St Gallan, Res Dept, St Gallen, Switzerland
[2] Univ Bristol, Sch Med & Vet Sci, Dept Pathol & Microbiol, Bristol BS8 1TH, Avon, England
来源
CORONAVIRUS REPLICATION AND REVERSE GENETICS | 2005年 / 287卷
关键词
D O I
10.1007/3-540-26765-4_7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In this article, we describe the reverse genetic system that is based on the use of vaccinia virus cloning vectors. This system represents a generic approach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229E representing a group I coronavirus. Subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse hepatitis virus, representing group III and group II coronaviruses, respectively. We describe how vaccinia virus-mediated homologous recombination can be used to introduce specific mutations into the coronavirus genomic cDNA during its propagation in vaccinia virus and how recombinant coronaviruses can be isolated. Finally, we describe how the coronavirus reverse genetic system has now been extended to the generation of coronavirus replicon RNAs.
引用
收藏
页码:199 / 227
页数:29
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