Micromechanical mass sensors for biomolecular detection in a physiological environment

被引：133

作者：

Braun, T ^{[1
]}

Barwich, V ^{[1
]}

Ghatkesar, MK ^{[1
]}

Bredekamp, AH ^{[1
]}

Gerber, C ^{[1
]}

Hegner, M ^{[1
]}

Lang, HP ^{[1
]}

机构：

[1] Univ Basel, Inst Phys, Natl Ctr Competence Res Nanosci, CH-4056 Basel, Switzerland

来源：

PHYSICAL REVIEW E | 2005年 / 72卷 / 03期

关键词：

D O I：

10.1103/PhysRevE.72.031907

中图分类号：

O35 [流体力学]; O53 [等离子体物理学];

学科分类号：

070204 ; 080103 ; 080704 ;

摘要：

Micromechanical cantilever arrays are used to measure time-resolved adsorption of tiny masses based on protein-ligand interactions. Here, streptavidin-biotin interactions are investigated in a physiological environment. A measurement method is introduced using higher flexural modes of a silicon cantilever in order to enhance the sensitivity of mass detection. Modeling the cantilever vibration in liquid allows the measurement of absolute mass changes. We show time-resolved mass adsorption of final 7 +/- 0.7 ng biotinylated latex beads. The sensitivity obtained is about 2.5 pg/Hz measuring at a center frequency of 750 kHz.

引用

页数：9

共 27 条

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