An in situ electrochemical surface plasmon resonance immunosensor with polypyrrole propylic acid film:: Comparison between SPR and electrochemical responses from polymer formation to protein immunosensing

被引：69

作者：

Dong, Hua ^{[1
]}

Cao, Xiaodong ^{[1
]}

Li, Chang Ming ^{[1
]}

Hu, Weihua ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Chem Biomed Engn, Ctr Adv Bionanosyst, Singapore 637457, Singapore

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 07期

关键词：

electrochemical surface plasmon resonance (EC-SPR); sandwich immunosensor; electropolymerization; amperometry;

D O I：

10.1016/j.bios.2007.10.026

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel in situ electrochemical surface plasmon resonance (EC-SPR) immunosensor is presented in this paper. The EC-SPR measurement can be used to in situ monitor the polymer formation, probe immobilization, antigen-antibody interaction and protein immunosensing process. A sandwich immunosensor based on permeable polypyrrole propylic acid (PPA) film is constructed using mouse IgG as a model analyte. The results show that the introduction of capture antibody conjugated enzyme not only enhances the current responses but also increases the SPR angle shift. The calibration curves of electrochemical (EC) and surface plasmon resonance (SPR) measurement exhibit a similar dependence on the bulk concentration of antigen. An approximate linear relationship can be obtained by plotting the data in semi-logarithmic reference frame. Compared with SPR, EC shows higher sensitivity with prolonged time. The in situ EC-SPR immunosensor described herein could have important potentials for diagnostics and medicine applications. (C) 2007 Published by Elsevier B.V.

引用

页码：1055 / 1062

页数：8

共 43 条

[11] Sensitive electrochemical enzyme immunoassay microdevice based on architecture of dual ring electrodes with a sensing cavity chamber [J].

Dong, H. ;

Li, C. M. ;

Zhou, Q. ;

Sun, J. B. ;

Miao, J. M. .

BIOSENSORS & BIOELECTRONICS, 2006, 22 (05) :621-626

[12] Sensitive amperometric immunosensing using polypyrrolepropylic acid films for biomolecule immobilization [J].

Dong, Hua ;

Li, Chang Ming ;

Chen, Wei ;

Zhou, Qin ;

Zeng, Zhao Xian ;

Luong, John H. T. .

ANALYTICAL CHEMISTRY, 2006, 78 (21) :7424-7431

[13] Characterization of self-assembled alkanethiol monolayers on silver and gold using surface plasmon spectroscopy [J].

Ehler, TT ;

Malmberg, N ;

Noe, LJ .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (08) :1268-1272

[14] Surface plasmon resonance spectroscopy study of electrostatically adsorbed layers [J].

Gandubert, VJ ;

Lennox, RB .

LANGMUIR, 2006, 22 (10) :4589-4593

[15] Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays [J].

Goodrich, TT ;

Lee, HJ ;

Corn, RM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (13) :4086-4087

[16] SURFACE-PLASMONS AS A PROBE OF THE ELECTROCHEMICAL INTERFACE [J].

GORDON, JG ;

ERNST, S .

SURFACE SCIENCE, 1980, 101 (1-3) :499-506

[17] Surface plasmon resonance spectroscopy and electrochemistry study of 4-nitro-1,2-phenylenediamine: A switchable redox polymer with nitro functional groups [J].

Gu, HR ;

Ng, ZY ;

Deivaraj, TC ;

Su, XD ;

Loh, KP .

LANGMUIR, 2006, 22 (08) :3929-3935

[18] Characterization and optimization of a real-time, parallel, label-free, polypyrrole-based DNA sensor by surface plasmon resonance imaging [J].

Guedon, P ;

Livache, T ;

Martin, F ;

Lesbre, F ;

Roget, A ;

Bidan, G ;

Levy, Y .

ANALYTICAL CHEMISTRY, 2000, 72 (24) :6003-6009

[19] In situ studies of protein adsorptions on poly(pyrrole-co-pyrrole propylic acid) film by electrochemical surface plasmon resonance [J].

hu, Weia Hu ;

Li, Chang Ming ;

Cui, Xiaoqiang ;

Dong, Hua ;

Zhou, Qin .

LANGMUIR, 2007, 23 (05) :2761-2767

[20] Detection of electrochemical enzymatic reactions by surface plasmon resonance measurement [J].

Iwasaki, Y ;

Horiuchi, T ;

Niwa, O .

ANALYTICAL CHEMISTRY, 2001, 73 (07) :1595-1598

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