Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets

被引:134
作者
Bai, Lijuan [1 ]
Yuan, Ruo [1 ]
Chai, Yaqin [1 ]
Zhuo, Ying [1 ]
Yuan, Yali [1 ]
Wang, Yan [1 ]
机构
[1] Southwest Univ, Educ Minist, Key Lab Luminescence & Real Time Anal, Coll Chem & Chem Engn, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
Electrochemical aptasensor; Simultaneous detection; Signal amplification; Multi-labeled graphene sheets; GOLD NANOPARTICLES; THROMBIN DETECTION; FACILE SYNTHESIS; DNA DETECTION; APTAMER; APTASENSOR; BIOSENSOR; GROWTH; NANOELECTROCATALYST; REAGENTLESS;
D O I
10.1016/j.biomaterials.2011.10.012
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this work, a sandwich-type electrochemical aptasensor for simultaneous sensitive detection of platelet-derived growth factor (PDGF) and thrombin is fabricated. Reduced graphene oxide sheets (rGS) are used as matrices to immobilize the redox probes, which are subsequently coated with platinum nanoparticles (PtNPs) to form the PtNPs-redox probes-rGS nanocomposites. With the employment of the as prepared nanocomposites, a signal amplification strategy was described based on bienzyme (glucose oxidase and horseradish peroxidase) modified PtNPs-redox probes-rGS nanocomposites as the tracer labels for secondary aptamers (Apt II) through sandwiched assay. Gold nanoparticles functionalized single-walled carbon nanotubes (AuNPs@SWCNTs) as the biosensor platform enhance the surface area to capture a large amount of primary aptamers (Apt I), thus amplifying the detection response. The experiment results show that the multi-labeled PtNPs-redox probes-rGS nanocomposites display satisfying electrochemical redox activity and highly electrocatalytic activity of PtNPs and bienzyme, which exhibit high sensitivity for detection of proteins. The linear range of PDGF is 0.01-35 nM with a detection limit of 8 pM, while the linear ranges from 0.02 to 45 nM and a detection limit of 11 pM for thrombin are obtained. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1090 / 1096
页数:7
相关论文
共 33 条
[1]   Ultrasensitive and selective DNA detection based on nicking endonuclease assisted signal amplification and its application in cancer cell detection [J].
Bi, Sai ;
Zhang, Jilei ;
Zhang, Shusheng .
CHEMICAL COMMUNICATIONS, 2010, 46 (30) :5509-5511
[2]   In situ Controllable Growth of Prussian Blue Nanocubes on Reduced Graphene Oxide: Facile Synthesis and Their Application as Enhanced Nanoelectrocatalyst for H2O2 Reduction [J].
Cao, Linyuan ;
Liu, Yanlan ;
Zhang, Baohua ;
Lu, Lehui .
ACS APPLIED MATERIALS & INTERFACES, 2010, 2 (08) :2339-2346
[3]   Graphene Fluorescence Resonance Energy Transfer Aptasensor for the Thrombin Detection [J].
Chang, Haixin ;
Tang, Longhua ;
Wang, Ying ;
Jiang, Jianhui ;
Li, Jinghong .
ANALYTICAL CHEMISTRY, 2010, 82 (06) :2341-2346
[4]   Impedimetric Aptasensor with Femtomolar Sensitivity Based on the Enlargement of Surface-Charged Gold Nanoparticles [J].
Deng, Chunyan ;
Chen, Jinhua ;
Nie, Zhou ;
Wang, Mengdong ;
Chu, Xiaochen ;
Chen, Xiaoli ;
Xiao, Xilin ;
Lei, Chunyang ;
Yao, Shouzhuo .
ANALYTICAL CHEMISTRY, 2009, 81 (02) :739-745
[5]   Graphene Oxide: A Convenient Carbocatalyst for Facilitating Oxidation and Hydration Reactions [J].
Dreyer, Daniel R. ;
Jia, Hong-Peng ;
Bielawski, Christopher W. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (38) :6813-6816
[6]   Multifunctional label-free electrochemical biosensor based on an integrated aptamer [J].
Du, Yan ;
Li, Bingling ;
Wei, Hui ;
Wang, Yuling ;
Wang, Erkang .
ANALYTICAL CHEMISTRY, 2008, 80 (13) :5110-5117
[7]   Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination [J].
Du, Yan ;
Chen, Chaogui ;
Li, Bingling ;
Zhou, Ming ;
Wang, Erkang ;
Dong, Shaojun .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (08) :1902-1907
[8]   A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells [J].
Feng, Lingyan ;
Chen, Yong ;
Ren, Jinsong ;
Qu, Xiaogang .
BIOMATERIALS, 2011, 32 (11) :2930-2937
[9]   Three-Dimensional Pt-on-Pd Bimetallic Nanodendrites Supported on Graphene Nanosheet: Facile Synthesis and Used as an Advanced Nanoelectrocatalyst for Methanol Oxidation [J].
Guo, Shaojun ;
Dong, Shaojun ;
Wang, Erkang .
ACS NANO, 2010, 4 (01) :547-555
[10]   A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis [J].
He, Shijiang ;
Song, Bo ;
Li, Di ;
Zhu, Changfeng ;
Qi, Wenpeng ;
Wen, Yanqin ;
Wang, Lihua ;
Song, Shiping ;
Fang, Haiping ;
Fan, Chunhai .
ADVANCED FUNCTIONAL MATERIALS, 2010, 20 (03) :453-459