Evaluation of tumoral enhancement by superparamagnetic iron oxide particles:: comparative studies with ferumoxtran and anionic iron oxide nanoparticles

被引：22

作者：

Brillet, PY ^{[1
]}

Gazeau, F ^{[1
]}

Luciani, A ^{[1
]}

Bessoud, B ^{[1
]}

Cuénod, CA ^{[1
]}

Siauve, N ^{[1
]}

Pons, JN ^{[1
]}

Poupon, J ^{[1
]}

Clément, O ^{[1
]}

机构：

[1] Hop Avicenne, Dept Radiol, F-93009 Bobigny, France

来源：

EUROPEAN RADIOLOGY | 2005年 / 15卷 / 07期

关键词：

iron oxide nanoparticles; relaxometry; magnetic resonance imaging; cell magnetic labeling; tumor targeting;

D O I：

10.1007/s00330-004-2586-8

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

This study was designed to compare tumor enhancement by superparamagnetic iron oxide particles, using anionic iron oxide nanoparticles (AP) and ferumoxtran. In vitro, relaxometry and media with increasing complexity were used to assess the changes in r2 relaxivity due to cellular internalization. In vivo, 26 mice with subcutaneously implanted tumors were imaged for 24 h after injection of particles to describe kinetics of enhancement using T1 spin echo, T2 spin echo, and T2 fast spin echo sequences. In vitro, the r2 relaxivity decreased over time (0-4 h) when AP were uptaken by cells. The loss of r2 relaxivity was less pronounced with long (Hahn Echo) than short (Carr-Purcell-Meiboom-Gill) echo time sequences. In vivo, our results with ferumoxtran showed an early T2 peak (1 h), suggesting intravascular particles and a second peak in T1 (12 h), suggesting intrainterstitial accumulation of particles. With AP, the late peak (24 h) suggested an intracellular accumulation of particles. In vitro, anionic iron oxide nanoparticles are suitable for cellular labeling due to a high cellular uptake. Conversely, in vivo, ferumoxtran is suitable for passive targeting of tumors due to a favorable biodistribution.

引用

页码：1369 / 1377

页数：9

共 38 条

[1] Ex vivo cell labeling with 64Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography [J].