Fluorescence lifetime imaging in an optically sectioning programmable array microscope (PAM)

被引：18

作者：

Hanley, QS ^{[1
]}

Lidke, KA ^{[1
]}

Heintzmann, R ^{[1
]}

Arndt-Jovin, DJ ^{[1
]}

Jovin, TM ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany

来源：

CYTOMETRY PART A | 2005年 / 67A卷 / 02期

关键词：

frequency domain lifetime imaging; AB plot; structured illumination;

D O I：

10.1002/cyto.a.20177

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: The programmable array microscopes (PAMs) are a family of instruments incorporating arbitrary control of the patterns of illumination and/or detection. The PAM can be used in sectioning and nonsectioning modes, thereby constituting a useful platform for fluorescence lifetime imaging. Methods and Results: We used a PAM for acquisition of optically sectioned and widefield fluorescence lifetime images, in which contrast was increased predominantly by suppressing out-of-focus light contributions. We simulate, display, and discuss the effects of blurring and fluorophore heterogeneity on lifetime imaging in widefield and confocal configurations. Conclusion: Sectioning improves the quality of lifetime images of samples with multiple fluorophores or spatially varying Forster resonance energy transfer. (c) 2005 International Society for Analytical Cytology.

引用

页码：112 / 118

页数：7

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