Preparation and characterization of hydroxypropyl chitosan modified with collagen peptide

被引：39

作者：

Fan, Lihong ^{[1
]}

Zou, Shengqiong ^{[1
]}

Ge, Hongyu ^{[1
]}

Xiao, Yao ^{[1
]}

Wen, Huigao ^{[1
]}

Feng, Han ^{[1
]}

Liu, Meng ^{[1
]}

Nie, Min ^{[2
,3
]}

机构：

[1] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Peoples R China

[2] Wuhan Univ, State Key Lab Breeding Base Basic Sci Stomatol Hu, Minist Educ, Sch & Hosp Stomatol, Wuhan 430079, Peoples R China

[3] Wuhan Univ, Key Lab Oral Biomed, Minist Educ, Sch & Hosp Stomatol, Wuhan 430079, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 93卷

基金：

中国国家自然科学基金;

关键词：

Collagen peptide; Hydroxypropyl chitosan; Microbial transglutaminase; Wound dressing; RADICAL-SCAVENGING ACTIVITY; MICROBIAL TRANSGLUTAMINASE; DRUG-DELIVERY; CROSS-LINKING; GELATIN; GEL;

D O I：

10.1016/j.ijbiomac.2016.07.093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Collagen peptide (COP) had been grafted to hydroxypropyl chitosan (HPCS) by using microbial transglutaminase (MTGase) as biocatalyst. HPCS was synthesized from chitosan and propylene oxide under alkali condition. The chemical structures of derivative were characterized by FT-IR and H-1 NMR spectroscopy. The process conditions were optimized from the aspects of the reaction time, the reaction temperature, the molar ratio of COP and the mass ratio of MTGase to HPCS. In this study, HPCS-COP could serve not only to reduce the loss of moisture but also to absorb the moisture, and the moisture absorption and moisture retention abilities were closely related to the degree of substitution (DS) values. In addition, with the DS and concentration increase of HPCS-COP, the radical scavenging activity increased in vitro antioxidant activity. Furthermore, the methyl thiazolyl tetrazolium assay (MTT) was applied to evaluate the biocompatibility of HPCS-COP, and the result indicated that HPCS-COP with the DS of 0.34 displayed pronounced cell viability at 50 ppm. Therefore, the results suggest that HPCS-COP could be potential wound dressings for clinical applications. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：636 / 643

页数：8

共 36 条

[1]

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