Trranscript profiling of a conifer pathosystem:: response of Pinus sylvestris root tissues to pathogen (Heterobasidion annosum) invasion

被引:53
作者
Adomas, Aleksandra
Heller, Gregory
Li, Guosheng
Olson, Ake
Chu, Tzu-Ming
Osborne, Jason
Craig, Deborah
Van Zyl, Len
Wolfinger, Russ
Sederoff, Ron
Dean, Ralph A.
Stenlid, Jan
Finlay, Roger
Asiegbu, Frederick O.
机构
[1] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, Uppsala, Sweden
[2] SAS Inst, Cary, NC 27513 USA
[3] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA
[4] N Carolina State Univ, Forest Biotechnol Lab, Raleigh, NC 27695 USA
[5] N Carolina State Univ, Fungal Genom Lab, Raleigh, NC 27695 USA
关键词
antimicrobial peptide; microarray; peroxidase; phenylopropanoid pathway; pine; plant defence;
D O I
10.1093/treephys/27.10.1441
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
The mechanisms underlying defence reactions to a pathogen attack, though well studied in crop plants, are poorly understood in conifers. To analyze changes in gene transcript abundance in Pinus sylvestris L. root tissues infected by Heterobasidion annosum (Fr.) Bref. s.l., a cDNA microarray containing 2109 ESTs from P. taeda L. was used. Mixed model statistical analysis identified 179 expressed sequence tags differentially expressed at 1, 5 or 15 days post inoculation. In general, the total number of genes differentially expressed during the infection increased over time. The most abundant group of genes up-regulated upon infection coded for enzymes involved in metabolism (phenylpropanoid pathway) and defence-related proteins with antimicrobial properties. A class III peroxidase responsible for lignin biosynthesis and cell wall thickening had increased transcript abundance at all measurement times. Real-time RT-PCR verified the microarray results with high reproducibility. The similarity of the expression profiling pattern observed in this pathosystem to those documented in crop pathology suggests that angiosperms and gymnosperms use similar genetic programs in responding to invasive growth by microbial pathogens.
引用
收藏
页码:1441 / 1458
页数:18
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