Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples

被引：42

作者：

Kreuzer, MP ^{[1
]}

O'Sullivan, CK ^{[1
]}

Pravda, M ^{[1
]}

Guilbault, GG ^{[1
]}

机构：

[1] Natl Univ Ireland, Dept Analyt Chem, Lab Sensor Dev, Cork, Ireland

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 442卷 / 01期

关键词：

allergy; IgE; ELISA; screen-printed electrode (SPE); biosensor; blood;

D O I：

10.1016/S0003-2670(01)01137-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml(-1) IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：45 / 53

页数：9

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