Effects of a bile acid elicitor, cholic acid, on the biosynthesis of diterpenoid phytoalexins in suspension-cultured rice cells

被引：66

作者：

Shimizu, Takafumi ^{[1
]}

Jikumaru, Yusuke ^{[1
]}

Okada, Atsushi ^{[1
]}

Okada, Kazunori ^{[1
]}

Koga, Jinichiro ^{[2
]}

Umemura, Kenji ^{[3
]}

Minami, Eiichi ^{[4
]}

Shibuya, Naoto ^{[5
]}

Hasegawa, Morifumi ^{[6
]}

Kodama, Osamu ^{[6
]}

Nojiri, Hideaki ^{[1
]}

Yamane, Hisakazu ^{[1
]}

机构：

[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1148657, Japan

[2] Meiji Seika Kaisha Ltd, Food & Hlth R&D Labs, Sakado, Saitama 3500289, Japan

[3] Meiji Seika Kaisha Ltd, Agr & Vet Res Labs, Kohoku Ku, Yokohama, Kanagawa 2228567, Japan

[4] Natl Inst Agrobiol Sci, Dept Biochem, Tsukuba, Ibaraki 3058602, Japan

[5] Meiji Univ, Dept Life Sci, Sch Agr, Kanagawa 2148571, Japan

[6] Ibaraki Univ, Coll Agr, Ami, Ibaraki 3000393, Japan

来源：

PHYTOCHEMISTRY | 2008年 / 69卷 / 04期

关键词：

index Oryza sativa; gramineae; rice suspension cells; phytoalexin; phytocassane; diterpene cyclase gene; cholic acid; HPLC-MS/MS;

D O I：

10.1016/j.phytochem.2007.10.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An elicitor of rice defense responses was recently isolated from human feces and was identified as cholic acid (CA). Pathogen infection in rice leaves induces phytocassanes and momilactones, both of which are major diterpenoid phytoalexins in rice, whereas CA mainly induces phytocassanes. We established a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry protocol for the rapid and accurate quantification of phytocassanes and momilactones. Using this method, we showed that CA preferentially induced the formation of phytocassanes in suspension-cultured rice cells, while a fungal chitin oligosaccharide elicitor induced that of both phytocassanes and momilactones. We further investigated the effects of CA on the expression of diterpene cyclase genes involved in phytoalexin biosynthesis. CA induced the transcription of the genes OsCPS2 (OsCyc2) and OsKSL7 (OsDTC1), which are involved in phytocassane biosynthesis, to a greater extent than the genes OsCPS4 (OsCyc1) and OsKSL4, which are involved in momilactone biosynthesis. OsCPS2 was particularly strongly induced, suggesting that it is one of the main mechanisms by which CA induces high levels of phytocassanes. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：973 / 981

页数：9

共 18 条

[1] Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor [J].

Cho, EM ;

Okada, A ;

Kenmoku, H ;

Otomo, K ;

Toyomasu, T ;

Mitsuhashi, W ;

Sassa, T ;

Yajima, A ;

Yabuta, G ;

Mori, K ;

Oikawa, H ;

Toshima, H ;

Shibuya, N ;

Nojiri, H ;

Omori, T ;

Nishiyama, M ;

Yamane, H .

PLANT JOURNAL, 2004, 37 (01) :1-8

[2] Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice [J].

Chuio, Tetsuya ;

Takai, Ryota ;

Akimoto-Tomiyama, Chiharu ;

Ando, Sugihiro ;

Minami, Eiichi ;

Nagamura, Yoshiaki ;

Kaku, Hanae ;

Shibuya, Naoto ;

Yasuda, Michiko ;

Nakashita, Hideo ;

Umemura, Kenji ;

Okada, Atsushi ;

Okada, Kazunori ;

Nojiri, Hideaki ;

Yamane, Hisakazu .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2007, 1769 (7-8) :497-505

[3] Identification of a high-affinity binding protein for N-acetylchitooligosaccharide elicitor in the plasma membrane of suspension-cultured rice cells by affinity labeling [J].

Ito, Y ;

Kaku, H ;

Shibuya, N .

PLANT JOURNAL, 1997, 12 (02) :347-356

[4] Cholic acid, a bile acid elicitor of hypersensitive cell death, pathogenesis-related protein synthesis, and phytoalexin accumulation in rice [J].

Koga, J ;

Kubota, H ;

Gomi, S ;

Umemura, K ;

Ohnishi, M ;

Kono, T .

PLANT PHYSIOLOGY, 2006, 140 (04) :1475-1483

[5] Functional moiety for the antifungal activity of phytocassane E, a diterpene phytoalexin from rice [J].

Koga, J ;

Ogawa, N ;

Yamauchi, T ;

Kikuchi, M ;

Ogasawara, N ;

Shimura, M .

PHYTOCHEMISTRY, 1997, 44 (02) :249-253

[6] PHYTOCASSANE-A, PHYTOCASSANE-B, PHYTOCASSANE-C AND PHYTOCASSANE-D, NOVEL DITERPENE PHYTOALEXINS FROM RICE, ORYZA-SATIVA L [J].

KOGA, J ;

SHIMURA, M ;

OSHIMA, K ;

OGAWA, N ;

YAMAUCHI, T ;

OGASAWARA, N .

TETRAHEDRON, 1995, 51 (29) :7907-7918

[7] Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice [J].

Nemoto, T ;

Cho, EM ;

Okada, A ;

Okada, K ;

Otomo, K ;

Kanno, Y ;

Toyomasu, T ;

Mitsuhashi, W ;

Sassa, T ;

Minami, E ;

Shibuya, N ;

Nishiyama, M ;

Nojiri, H ;

Yamane, H .

FEBS LETTERS, 2004, 571 (1-3) :182-186

[8] Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells [J].

Nojiri, H ;

Sugimori, M ;

Yamane, H ;

Nishimura, Y ;

Yamada, A ;

Shibuya, N ;

Kodama, O ;

Murofushi, N ;

Omori, T .

PLANT PHYSIOLOGY, 1996, 110 (02) :387-392

[9] Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice [J].

Okada, Atsushi ;

Shimizu, Takafumi ;

Okada, Kazunori ;

Kuzuyama, Tomohisa ;

Koga, Jinichiro ;

Shibuya, Naoto ;

Nojiri, Hideaki ;

Yamane, Hisakazu .

PLANT MOLECULAR BIOLOGY, 2007, 65 (1-2) :177-187

[10] Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice [J].

Otomo, K ;

Kanno, Y ;

Motegi, A ;

Kenmoku, H ;

Yamane, H ;

Mitsuhashi, W ;

Kawa, H ;

Toshima, H ;

Itoh, H ;

Matsuoka, M ;

Sassa, T ;

Toyomasu, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (09) :2001-2006

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