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Comparison of the performance characteristics of poly(dimethylsiloxane) and Pyrex microchip electrophoresis devices for peptide separations

被引：73
作者：
Lacher, NA
de Rooij, NF
Verpoorte, E
Lunte, SM
机构：
[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA
[2] Univ Neuchatel, Inst Microtechnol, Sensors Actuators & Microsyst Lab, CH-2000 Neuchatel, Switzerland
来源：
JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1004卷 / 1-2期
关键词：
chip technology; microfluidics; instrumentation; poly(dimethylsiloxane); Pyrex; peptides;
D O I：
10.1016/S0021-9673(03)00722-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comparative study of electrophoretic separations of fluorescently labeled peptides and amino acids on poly(dimethylsiloxane) (PDMS) and Pyrex microchips is presented. The separation parameters for each microchip substrate were compared, including electroosmotic flow, plate numbers, resolution, and limits of detection. The effect of buffer composition on the separation was also investigated. Acceptable separations were obtained for most peptides with both substrates; however, PDMS chips exhibited much lower separation efficiencies and longer analysis times. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：225 / 235
页数：11
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