Microchip structures for submillisecond electrophoresis

被引：277

作者：

Jacobson, SC ^{[1
]}

Culbertson, CT ^{[1
]}

Daler, JE ^{[1
]}

Ramsey, JM ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 16期

关键词：

D O I：

10.1021/ac980349t

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microchip electrophoresis was used to resolve a binary mixture in 0.8 ms using a separation field strength of 53 kV cm(-1) and a separation length of 200 mu m. The microchip design permitted an electric field strength of 6.1 V cm(-1) in the separation channel per volt applied to the microchip. Concurrently, the spatial extent of the injection plug and the detector observation region were minimized to increase separation efficiency.

引用

收藏

页码：3476 / 3480

页数：5

相关论文

共 31 条

[1]

BURGGRAF N, 1993, HRC-J HIGH RES CHROM, V16, P594

[2] ELECTROOSMOSIS - A RELIABLE FLUID PROPULSION SYSTEM FOR FLOW-INJECTION ANALYSIS [J].

DASGUPTA, PK ;

LIU, S .

ANALYTICAL CHEMISTRY, 1994, 66 (11) :1792-1798

[3] HIGH-SPEED SEPARATION OF ANTISENSE OLIGONUCLEOTIDES ON A MICROMACHINED CAPILLARY ELECTROPHORESIS DEVICE [J].

EFFENHAUSER, CS ;

PAULUS, A ;

MANZ, A ;

WIDMER, HM .

ANALYTICAL CHEMISTRY, 1994, 66 (18) :2949-2953

[4] GLASS CHIPS FOR HIGH-SPEED CAPILLARY ELECTROPHORESIS SEPARATIONS WITH SUBMICROMETER PLATE HEIGHTS [J].

EFFENHAUSER, CS ;

MANZ, A ;

WIDMER, HM .

ANALYTICAL CHEMISTRY, 1993, 65 (19) :2637-2642

[5] Integrated capillary electrophoresis devices with an efficient postcolumn reactor in planar quartz and glass chips [J].

Fluri, K ;

Fitzpatrick, G ;

Chiem, N ;

Harrison, DJ .

ANALYTICAL CHEMISTRY, 1996, 68 (23) :4285-4290

[6]

Giddings J.C., 1991, Unified separation science

[7] Microchip device for performing enzyme assays [J].

Hadd, AG ;

Raymond, DE ;

Halliwell, JW ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1997, 69 (17) :3407-3412

[8] MICROMACHINING A MINIATURIZED CAPILLARY ELECTROPHORESIS-BASED CHEMICAL-ANALYSIS SYSTEM ON A CHIP [J].

HARRISON, DJ ;

FLURI, K ;

SEILER, K ;

FAN, ZH ;

EFFENHAUSER, CS ;

MANZ, A .

SCIENCE, 1993, 261 (5123) :895-897

[9] CAPILLARY ELECTROPHORESIS AND SAMPLE INJECTION SYSTEMS INTEGRATED ON A PLANAR GLASS CHIP [J].

HARRISON, DJ ;

MANZ, A ;

FAN, ZH ;

LUDI, H ;

WIDMER, HM .

ANALYTICAL CHEMISTRY, 1992, 64 (17) :1926-1932

[10] Integrated microdevice for DNA restriction fragment analysis [J].

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1996, 68 (05) :720-723

← 1 2 3 4 →