Disruption of γ-glutamylcysteine synthetase results in absolute glutathione auxotrophy and apoptosis in Candida albicans

被引：50

作者：

Baek, YU

Kim, YR

Yim, HS ^{[1
]}

Kang, SO

机构：

[1] Seoul Natl Univ, Sch Biol Sci, Biophys Lab, Seoul 151742, South Korea

[2] Seoul Natl Univ, Inst Microbiol, Seoul 151742, South Korea

来源：

FEBS LETTERS | 2004年 / 556卷 / 1-3期

关键词：

glutathione; gamma-glutamylcysteine synthetase; apoptosis; Candida albicans;

D O I：

10.1016/S0014-5793(03)01363-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Glutathione is the most abundant non-protein thiol and a major source of reducing equivalents in eukaryotes. We examined the role of glutathione in Candida albicans by the disruption of gamma-glutamylcysteine synthetase (GCS1), an essential enzyme in glutathione biosynthesis. The gcs1lgcs1 null mutants exhibited glutathione auxotrophy, which could be rescued by supplementing with reduced and oxidized glutathione and gamma-glutamylcysteine. When the mutants were depleted of glutathione, they showed typical markers of apoptosis. These results suggest that glutathione itself is an essential metabolite and C. albicans lacking GCS1 undergoes apoptosis. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：47 / 52

页数：6

共 38 条

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ISOLATION AND INITIAL CHARACTERIZATION OF GLUTATHIONE-DEFICIENT MUTANTS OF ESCHERICHIA-COLI K-12 [J].