Virus fitness differences observed between two naturally occurring isolates of Ebola virus Makona variant using a reverse genetics approach

被引：9

作者：

Albarino, Cesar G. ^{[1
]}

Guerrero, Lisa Wiggleton ^{[1
]}

Chakrabarti, Ayan K. ^{[1
]}

Kainulainen, Markus H. ^{[1
]}

Whitmer, Shannon L. M. ^{[1
]}

Welch, Stephen R. ^{[1
]}

Nichol, Stuart T. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, 1600 Clifton Rd, Atlanta, GA 30329 USA

来源：

VIROLOGY | 2016年 / 496卷

关键词：

Ebola virus; West Africa outbreak; Makona variant; Recombinant virus; Reverse genetics; SUBTYPE RESTON VIRUS; VP30; TRANSCRIPTION; REPLICATION; EVOLUTION; DISEASE; MARBURG; PHOSPHORYLATION; EPIDEMIOLOGY; TRANSMISSION;

D O I：

10.1016/j.virol.2016.06.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During the large outbreak of Ebola virus disease that occurred in Western Africa from late 2013 to early 2016, several hundred Ebola virus (EBOV) genomes have been sequenced and the virus genetic drift analyzed. In a previous report, we described an efficient reverse genetics system designed to generate recombinant EBOV based on a Makona variant isolate obtained in 2014. Using this system, we characterized the replication and fitness of 2 isolates of the Makona variant. These virus isolates are nearly identical at the genetic level, but have single amino acid differences in the VP30 and L proteins. The potential effects of these differences were tested using minigenomes and recombinant viruses. The results obtained with this approach are consistent with the role of VP30 and L as components of the EBOV RNA replication machinery. Moreover, the 2 isolates exhibited clear fitness differences in competitive growth assays. Published by Elsevier Inc.

引用

页码：237 / 243

页数：7

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