Nestin-GFP Transgene Reveals Neural Precursor Cells in Adult Skeletal Muscle

被引:70
作者
Birbrair, Alexander [1 ,2 ]
Wang, Zhong-Min [1 ]
Messi, Maria Laura [1 ]
Enikolopov, Grigori N. [3 ]
Delbono, Osvaldo [1 ,2 ]
机构
[1] Wake Forest Univ, Bowman Gray Sch Med, Dept Internal Med Gerontol, Winston Salem, NC 27106 USA
[2] Wake Forest Univ, Bowman Gray Sch Med, Neurosci Program, Winston Salem, NC 27103 USA
[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
来源
PLOS ONE | 2011年 / 6卷 / 02期
基金
美国国家卫生研究院;
关键词
CENTRAL-NERVOUS-SYSTEM; MESENCHYMAL STEM-CELLS; PROGENITOR CELLS; SATELLITE CELLS; BETA-TUBULIN; SPINAL-CORD; DIFFERENTIATION; NEUROGENESIS; MULTIPOTENT; EXPRESSION;
D O I
10.1371/journal.pone.0016816
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Therapy for neural lesions or degenerative diseases relies mainly on finding transplantable active precursor cells. Identifying them in peripheral tissues accessible for biopsy, outside the central nervous system, would circumvent the serious immunological and ethical concerns impeding cell therapy. Methodology/Principal Findings: In this study, we isolated neural progenitor cells in cultured adult skeletal muscle from transgenic mice in which nestin regulatory elements control GFP expression. These cells also expressed the early neural marker Tuj1 and light and heavy neurofilament but not S100 beta, indicating that they express typical neural but not Schwann cell markers. GFP+/Tuj1+ cells were also negative for the endothelial and pericyte markers CD31 and alpha-smooth muscle actin, respectively. We established their a) functional response to glutamate in patch-clamp recordings; b) interstitial mesenchymal origin; c) replicative capacity; and d) the environment necessary for their survival after fluorescence-activated cell sorting. Conclusions/Significance: We propose that the decline in nestin-GFP expression in muscle progenitor cells and its persistence in neural precursor cells in muscle cultures provide an invaluable tool for isolating a population of predifferentiated neural cells with therapeutic potential.
引用
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页数:15
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