Aptamer-based molecular recognition for biosensor development

被引：129

作者：

Zhou, Jing ^{[1
]}

Battig, Mark R. ^{[1
]}

Wang, Yong ^{[1
]}

机构：

[1] Univ Connecticut, Dept Chem Mat & Biomol Engn, Storrs, CT 06269 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 398卷 / 06期

基金：

美国国家科学基金会;

关键词：

Molecular recognition; Biosensor; Ultrasensitive detection; Nucleic acid aptamer; Upstream aptamer selection; Downstream aptamer truncation; IN-VITRO SELECTION; SURFACE-PLASMON RESONANCE; ROLLING CIRCLE AMPLIFICATION; DNA APTAMERS; RNA APTAMERS; ELECTROCHEMICAL DETECTION; EARLY-DIAGNOSIS; BLOOD-SERUM; PROTEIN; BINDING;

D O I：

10.1007/s00216-010-3987-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleic acid aptamers are an emerging class of synthetic ligands and have recently attracted significant attention in numerous fields. One is in biosensor development. In principle, nucleic acid aptamers can be discovered to recognize any molecule of interest with high affinity and specificity. In addition, unlike most ligands evolved in nature, synthetic nucleic acid aptamers are usually tolerant of harsh chemical, physical, and biological conditions. These distinguished characteristics make aptamers attractive molecular recognition ligands for biosensing applications. This review first concisely introduces methods for aptamer discovery including upstream selection and downstream truncation, then discusses aptamer-based biosensor development from the viewpoint of signal production.

引用

页码：2471 / 2480

页数：10

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