Improved peptide sequencing using isotope information inherent in tandem mass spectra

被引：13

作者：

Cannon, WR ^{[1
]}

Jarman, KD

机构：

[1] Pacific NW Natl Lab, Computat Biosci, Richland, WA 99352 USA

[2] Pacific NW Natl Lab, Appl Math, Richland, WA 99352 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2003年 / 17卷 / 15期

关键词：

D O I：

10.1002/rcm.1119

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate here the use of natural isotopic 'labels' in peptides to aid in the identification of peptides with a de novo algorithm. Using data from ion trap tandem mass spectrometric (MS/MS) analysis of 102 tryptic peptides, we have analyzed multiple series of peaks within LCQ MS/MS spectra that 'spell' peptide sequences. Isotopic peaks from naturally abundant isotopes are particularly prominent even after peak centroiding on y- and b-series ions and lead to increased confidence in the identification of the precursor peptides. Sequence analysis of the MS/MS data is accomplished by finding sequences and subsequences in a hierarchical manner within the spectra. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：1793 / 1801

页数：9

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