Resistance of colorectal cancer cells to radiation and 5-FU is associated with MELK expression

被引:59
作者
Choi, Seungho [1 ]
Ku, Ja-Lok [1 ]
机构
[1] Seoul Natl Univ, Coll Med, Canc Res Inst, Cell Biol Lab, Seoul 110799, South Korea
基金
新加坡国家研究基金会;
关键词
Rectal cancer; Chemoradiotherapy; Cancer stem cell; MELK; Drug-resistance; Radio-resistance; LEUCINE-ZIPPER KINASE; PRO-APOPTOTIC MEMBER; PREOPERATIVE RADIOTHERAPY; STEM-CELLS; BCL-G; CYCLE; 5-FLUOROURACIL;
D O I
10.1016/j.bbrc.2011.07.060
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It was was reported that the local recurrence would be caused by cancer stem cells acquiring chemo-and radio-resistance. Recently, one of the potential therapeutic targets for colorectal and other cancers has been identified, which is maternal embryonic leucine zipper kinase (MELK). MELK is known as an embryonic and neural stem cell marker, and associated with the cell survival, cell proliferation, and apoptosis. In this study, SNU-503, which is a rectal cancer cell line, was treated with radiation or 5-fluorouracil (5-FU), and elevation of the MELK expression level was observed. Furthermore, the cell line was pre-treated with small interfering RNA (siRNA) against MELK mRNA before treatment of radiation or 5-FU and its effects on cell cycle and proliferation were observed. We demonstrated that knockdown of MELK reduced the proliferation of cells with radiation or 5-FU treatment. In addition, MELK suppression caused changes in cell cycle. In conclusion, MELK could be associated with increased resistance of colorectal cancer cells against radiation and 5-FU. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:207 / 213
页数:7
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