Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF

被引：98

作者：

Macounová, K ^{[1
]}

Cabrera, CR ^{[1
]}

Yager, P ^{[1
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 07期

关键词：

D O I：

10.1021/ac001013y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The micro-channels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 mm. The properties of pH gradients formed as a result of the electrolysis of water were influenced by variation of parameters such as the initial pH, ionic strength, and flow rate. Transverse IEF in pressure-driven flow is demonstrated using bovine serum albumin in a single ampholyte buffer as well as in multiple-component buffers, Experimental results of protein focusing compare well to predictions of a mathematical model. Optimal conditions for efficient continuous fractionation of a protein mixture are summarized and discussed.

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收藏

页码：1627 / 1633

页数：7

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