Editing plant genomes with CRISPR/Cas9

被引：335

作者：

Belhaj, Khaoula ^{[1
]}

Chaparro-Garcia, Angela ^{[1
]}

Kamoun, Sophien ^{[1
]}

Patron, Nicola J. ^{[1
]}

Nekrasov, Vladimir ^{[1
]}

机构：

[1] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 32卷

基金：

英国生物技术与生命科学研究理事会; 欧洲研究理事会;

关键词：

TARGETED MUTAGENESIS; HUMAN-CELLS; GUIDE RNA; HOMOLOGOUS RECOMBINATION; ANALYSIS REVEALS; CAS9; GENE; SPECIFICITY; NUCLEASES; ENDONUCLEASE;

D O I：

10.1016/j.copbio.2014.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.

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页码：76 / 84

页数：9

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