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Directed evolution of an amine oxidase for the preparative deracemisation of cyclic secondary amines

被引：100
作者：
Carr, R
Alexeeva, M
Dawson, MJ
Gotor-Fernández, V
Humphrey, CE
Turner, NJ
机构：
[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland
[2] Univ Hertfordshire, UH Innovat Ctr, Novacta Biosyst Ltd, Hatfield AL10 9AB, Herts, England
来源：
CHEMBIOCHEM | 2005年 / 6卷 / 04期
关键词：
amine oxidase; amines; biocatalysis; deracemisation; directed evolution; enantioselectivity;
D O I：
10.1002/cbic.200400329
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Off the rac. Directed evolution of the amine oxidase from Aspergillus niger (MAO-N), by using 1-methyltetrahydro-isoquinoline as the target substrate, led to the identification of a variant enzyme (Asn336Ser/Ile246Met) that possessed a greater Kcat value (5.5-fold) than the parent as well as high enantioselectivity (see scheme). This new variant was used for the deracemisation of cyclic secondary amines on a preparative scale. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：637 / 639
页数：3
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