Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates

被引：23

作者：

Niazov, Angelica ^{[1
]}

Freeman, Ronit ^{[1
]}

Girsh, Julia ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

来源：

SENSORS | 2011年 / 11卷 / 11期

关键词：

sensor; glucose; chemiluminescence; DNAzyme; quantum dots; hydrogen peroxide; G-quadruplex; chemiluminescence resonance energy transfer; SEMICONDUCTOR QUANTUM DOTS; COLORIMETRIC DETECTION; HEMIN/G-QUADRUPLEXES; THROMBIN APTASENSOR; AMPLIFIED DETECTION; TELOMERASE ACTIVITY; AU NANOPARTICLES; DNA; IONS; AMPLIFICATION;

D O I：

10.3390/s111110388

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.

引用

页码：10388 / 10397

页数：10

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