Proteomics analysis of rat brain postsynaptic density - Implications of the diverse protein functional groups for the integration of synaptic physiology

被引:207
作者
Li, KW
Hornshaw, MP
Van der Schors, RC
Watson, R
Tate, S
Casetta, B
Jimenez, CR
Gouwenberg, Y
Gundelfinger, ED
Smalla, KH
Smit, AB
机构
[1] Vrije Univ Amsterdam, Fac Earth & Life Sci, Neurosci Res Inst, Dept Mol & Cellular Neurobiol, NL-1081 HV Amsterdam, Netherlands
[2] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England
[3] Applera Italia, Appl Biosyst, I-20052 Monza, Italy
[4] Leibniz Inst Neurobiol, Dept Neurochem & Mol Biol, D-39118 Magdeburg, Germany
[5] FAN GmbH, D-39120 Magdeburg, Germany
关键词
D O I
10.1074/jbc.M303116200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The postsynaptic density contains multiple protein complexes that together relay the presynaptic neurotransmitter input to the activation of the postsynaptic neuron. In the present study we took two independent proteome approaches for the characterization of the protein complement of the postsynaptic density, namely 1) two-dimensional gel electrophoresis separation of proteins in conjunction with mass spectrometry to identify the tryptic peptides of the protein spots and 2) isolation of the trypsin-digested sample that was labeled with isotope-coded affinity tag, followed by liquid chromatography-tandem mass spectrometry for the partial separation and identification of the peptides, respectively. Functional grouping of the identified proteins indicates that the postsynaptic density is a structurally and functionally complex organelle that may be involved in a broad range of synaptic activities. These proteins include the receptors and ion channels for glutamate neurotransmission, proteins for maintenance and modulation of synaptic architecture, sorting and trafficking of membrane proteins, generation of anaerobic energy, scaffolding and signaling, local protein synthesis, and correct protein folding and breakdown of synaptic proteins. Together, these results imply that the postsynaptic density may have the ability to function (semi-)autonomously and may direct various cellular functions in order to integrate synaptic physiology.
引用
收藏
页码:987 / 1002
页数:16
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