Designer RNA-binding proteins New tools for manipulating the transcriptome

被引：35

作者：

Filipovska, Aleksandra

Rackham, Oliver ^{[1
]}

机构：

[1] Univ Western Australia, Western Australian Inst Med Res, Perth, WA 6009, Australia

来源：

RNA BIOLOGY | 2011年 / 8卷 / 06期

关键词：

RNA-protein interactions; splicing; RNA localization; translation; protein engineering; SINGLE-STRANDED RNA; MESSENGER-RNA; CRYSTAL-STRUCTURE; HUMAN PUMILIO; LIVING CELLS; IN-VITRO; GENE; RECOGNITION; SPECIFICITY; REVEALS;

D O I：

10.4161/rna.8.6.17907

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Post-transcriptional regulation of gene expression is ubiquitous and fundamental for the control of cell growth, differentiation and the complex developmental programs of multicellular eukaryotes. Despite this realization, the current tools that are available to study RNAs are limited in many respects. Recently we expanded the RNA recognition code of Pumilio and FBF homology (PUF) proteins, enabling RNA-binding proteins with programmable specificities to be designed. The design of proteins that can bind any RNA sequence of interest and modulate its function will be important to elucidate the mechanisms by which gene expression is controlled at the level of RNA and may provide potential therapeutics in the future.

引用

页码：978 / 983

页数：6

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[1] Sequence-specific binding of single-stranded RNA: is there a code for recognition? [J].