Magnetic immunoassays utilizing magnetic markers and a high-TC SQUID

被引：23

作者：

Enpuku, K ^{[1
]}

Inoue, K

Soejima, K

Yoshinaga, K

Kuma, H

Hamasaki, N

机构：

[1] Kyushu Univ, Res Inst Superconductor Sci & Syst, Fukuoka 8128581, Japan

[2] Kyushu Inst Technol, Dept Appl Chem, Kitakyushu, Fukuoka 8048550, Japan

[3] Kyushu Univ, Dept Med Sci, Fukuoka 8128582, Japan

来源：

IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY | 2005年 / 15卷 / 02期

关键词：

high-T-C SQUID; immunoassay; magnetic nanoparticle; remanence;

D O I：

10.1109/TASC.2005.849991

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Magnetic immunoassays utilizing magnetic markers and a high-T-c SQUID have been performed. We first showed a design of the SQUID for the sensitive detection of the magnetic signal from the marker, where the spatial distribution of the signal field was taken into account. Using the design, we can obtain the relationship between the magnetization of the maker and the signal flux detected with the SQUID. This relationship is important for quantitative evaluation of the immunoassay. Next, we detected biological materials called IgE with a remanence measurement method, where a marker made of Fe3O4 particles with diameter of 25 nm was used. We showed that 2 atto-mol of IgE can be detected, which shows high sensitivity of the present method.

引用

页码：660 / 663

页数：4

共 14 条

[1]

CANTOR R, 1995, SQUID SENSORS FUNDAM, P228

[2] Ultrasensitive magnetic biosensor for homogeneous immunoassay [J].

Chemla, YR ;

Crossman, HL ;

Poon, Y ;

McDermott, R ;

Stevens, R ;

Alper, MD ;

Clarke, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14268-14272

[3] Design of superconducting quantum interference device for magnetic immunoassays [J].

Enpuku, K ;

Ohba, AH ;

Inoue, K ;

Soejima, K .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 2004, 43 (9A) :6044-6049

[4] Biological immunoassay utilizing magnetic marker and high Tc superconducting quantum interference device magnetometer [J].

Enpuku, K ;

Kuroda, D ;

Ohba, A ;

Yang, TQ ;

Yoshinaga, K ;

Nakahara, T ;

Kuma, H ;

Hamasaki, N .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2003, 42 (12A) :L1436-L1438

[5] High Tc SQUID system and magnetic marker for biological immunoassays [J].

Enpuku, K ;

Kuroda, D ;

Yang, TQ ;

Yoshinaga, K .

IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY, 2003, 13 (02) :371-376

[6] Application of high Tc SQUID magnetometer to biological immunoassays [J].

Enpuku, K ;

Minotani, T ;

Hotta, M ;

Nakahodo, A .

IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY, 2001, 11 (01) :661-664

[7] Detection of magnetic nanoparticles with superconducting quantum interference device (SQUID) magnetometer and application to immunoassays [J].

Enpuku, K ;

Minotani, T ;

Gima, T ;

Kuroki, Y ;

Itoh, Y ;

Yamashita, M ;

Katakura, Y ;

Kuhara, S .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 1999, 38 (10A) :L1102-L1105

[8] Detection of bacteria in suspension by using a superconducting quantum interference device [J].

Grossman, HL ;

Myers, WR ;

Vreeland, VJ ;

Bruehl, R ;

Alper, MD ;

Bertozzi, CR ;

Clarke, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (01) :129-134

[9] Low Tc SQUID measurement system for magnetic relaxation immunoassays in unshielded environment [J].

Haller, A ;

Matz, H ;

Hartwig, S ;

Kerberger, T ;

Atzpadin, H ;

Trahms, L .

IEEE TRANSACTIONS ON APPLIED SUPERCONDUCTIVITY, 2001, 11 (01) :1371-1374

[10] Effective area in slotted high Tc dc washer superconducting quantum interference devices [J].

Jansman, ABM ;

Izquierdo, M ;

Eiguren, A ;

Flokstra, J ;

Rogalla, H .

APPLIED PHYSICS LETTERS, 1998, 72 (26) :3515-3517

← 1 2 →