Genetically engineered molecular networks for biosensing systems

被引：11

作者：

Aizawa, M ^{[1
]}

Yanagida, Y ^{[1
]}

Haruyama, T ^{[1
]}

Kobatake, E ^{[1
]}

机构：

[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 52卷 / 1-2期

关键词：

gene engineering; fusion protein; lipid-tagged antibody; TOL plasmid;

D O I：

10.1016/S0925-4005(98)00274-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gene engineering has been successfully applied to develop biosensing material, which includes an enzyme-labeled binding protein for enzyme immunosensing, a lipid-tagged single chain antibody for immunosensing and an environmentally responsive gene-bearing cell for environment sensing. protein A was labeled with firefly luciferase according to the gene engineering to fuse both proteins. A single chain antibody was genetically tagged with a lipid chain at a specific site by enzymatic modification of a preprotein in a transmembrane process within a bacterial cell. A recombinant plamid bearing xyl R protein and firefly luciferase genes with Ps promoter was transfected to E. coli to make the cell responsive to environmentally hazardous compounds such as xylene in emitting light. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：204 / 211

页数：8

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