Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits

被引：90

作者：

Das, Somes K.

Darshi, Manjula

Cheley, Stephen

Wallace, Mark I.

Bayley, Hagan

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

[2] Texas A&M Univ, Syst Hlth Sci Ctr, Dept Mol & Cellular Med, College Stn, TX 77843 USA

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 09期

基金：

英国医学研究理事会;

关键词：

alpha-hemolysin; fluorescence; leukocidin; photobleaching; single-molecule studies;

D O I：

10.1002/cbic.200600474

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Body count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. (Graph Presented). © 2007 Wiley-VCH Verlag GmbH&Co. KGaA.

引用

收藏

页码：994 / 999

页数：6

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