RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus

被引：262

作者：

Davis, E

Caiment, F

Tordoir, X

Cavaillé, J

Ferguson-Smith, A

Cockett, N

Georges, M

Charlier, C

机构：

[1] Univ Liege, Fac Vet Med, Dept Genet, B-4000 Liege, Belgium

[2] Univ Toulouse 3, IFR 109, UMR 5099, CNRS,Lab Biol Mol Eucaryote, F-31062 Toulouse, France

[3] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England

[4] Utah State Univ, Coll Agr, Logan, UT 84322 USA

来源：

CURRENT BIOLOGY | 2005年 / 15卷 / 08期

关键词：

D O I：

10.1016/j.cub.2005.02.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.

引用

页码：743 / 749

页数：7

共 16 条

[1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].