Non-viral gene transfection technologies for genetic engineering of stem cells

被引：50

作者：

Jo, Jun-Ichiro ^{[1
]}

Tabata, Yasuhiko ^{[1
]}

机构：

[1] Kyoto Univ, Dept Biomat, Kyoto, Japan

来源：

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | 2008年 / 68卷 / 01期

关键词：

non-viral gene carrier; genetic engineering; stem cells; drug delivery system; regenerative medical therapy;

D O I：

10.1016/j.ejpb.2007.04.021

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The recent rapid progress of molecular biology together with the steady progress of genome projects has given us some essential and revolutionary information about DNA and RNA to elucidate various biological phenomena at a genetic level. Under these circumstances, the technology and methodology of gene transfection have become more and more important to enhance the efficacy of gene therapy for several diseases. In addition, gene transfection is a fundamental technology indispensable to the further research development of basic biology and medicine regarding stem cells. Stem cells genetically manipulated will enhance the therapeutic efficacy of cell transplantation. In this paper, the carrier and technology of gene delivery are briefly overviewed while the applications to the basic researches of biology and medicine as well as regenerative medical therapy are introduced. A new non-viral carrier and the cell culture system are described to efficiently manipulate stem cells. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：90 / 104

页数：15

共 183 条

[1] Self-assembly of polymer amphiphiles: Thermodynamics of complexation between bovine serum albumin and self-aggregate of cholesterol-bearing pullulan [J].