A label-free immunosensor array using single-chain antibody fragments

被引：214

作者：

Backmann, N

Zahnd, C

Huber, F

Bietsch, A

Plückthun, A

Lang, HP

Güntherodt, HJ

Hegner, M

Gerber, C

机构：

[1] Univ Basel, Natl Ctr Competence Res Nanosci, Inst Phys, CH-4056 Basel, Switzerland

[2] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

[3] IBM Res, Zurich Res Lab, CH-8803 Ruschlikon, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 41期

关键词：

cantilever arrays; nanomechanics; proteomics;

D O I：

10.1073/pnas.0504917102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report a microcantilever-based immunosensor operated in static deflection mode with a performance comparable with surface plasmon resonance, using single-chain Fv (scFv) antibody fragments as receptor molecules. As a model system scFv fragments with specificity to two different antigens were applied. We introduced a cysteine residue at the C terminus of each scFv construct to allow covalent attachment to gold-coated sensor interfaces in directed orientation. Application of an array enabled simultaneous deflection measurements of sensing and reference cantilevers. The differential deflection signal revealed specific antigen binding and was proportional to the antigen concentration in solution. Using small, oriented scFv fragments as receptor molecules we increased the sensitivity of microcantilevers to approximate to 1 nM.

引用

收藏

页码：14587 / 14592

页数：6

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