Evaluation and application of the AmpF/STR Profiler Plus PCR amplification kit in a Bavarian population sample

被引：22

作者：

Anslinger, K ^{[1
]}

Rolf, B ^{[1
]}

Keil, W ^{[1
]}

机构：

[1] Univ Munich, Inst Legal Med, D-80337 Munich, Germany

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2001年 / 114卷 / 4-5期

关键词：

AmpF/STR Profiler Plus; STRs; Bavarian population; allelic drop out;

D O I：

10.1007/s004149900116

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Allele frequencies for the nine tetrameric STR loci D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 were determined in a population sample of 155 unrelated Bavarians using the AmpFlSTR Profiler Plus PCR amplification kit. No deviations from the Hardy-Weinberg equilibrium were observed. The influence of the PCR cycle number as well as the template DNA concentration on the performance of the kit was studied. DNA concentrations lower than 75 pg DNA per 25 mul reaction volume resulted in allelic drop-out.

引用

页码：278 / 280

页数：3

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