Human osteoblast response to pulsed laser deposited calcium phosphate coatings

被引：169

作者：

Bigi, A

Bracci, B

Cuisinier, F

Elkaim, R

Fini, M

Mayer, I

Mihailescu, IN

Socol, G

Sturba, L

Torricelli, P

机构：

[1] Univ Bologna, Dept Chem G Ciamician, I-40126 Bologna, Italy

[2] Inst Natl Sante & Rech Med, F-67085 Strasbourg, France

[3] Parogene Med Univ, F-67085 Strasbourg, France

[4] Res Inst Codivilla Putti Rizzoli Orthopaed Inst, Dept Expt Surg, Bologna, Italy

[5] Hebrew Univ Jerusalem, Dept Inorgan & Analyt Chem, IL-91904 Jerusalem, Israel

[6] Natl Inst Lasers Plasma & Radiat Phys, Bucharest 76900, Romania

来源：

BIOMATERIALS | 2005年 / 26卷 / 15期

关键词：

calcium phosphate coating; octacalcium phosphate; Mn2+-doped carbonated hydroxyapatite; laser ablation; human osteoblasts; differentiation;

D O I：

10.1016/j.biomaterials.2004.07.057

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Octacalcium phosphate (OCP) and Mn2+ -doped carbonate hydroxyapatite (Mn-CHA) thin films were deposited on pure, highly polished and chemically etched Ti substrates with pulsed laser depositiotn. The coatings exhibit different composition, crystallinity and morphology that might affect their osteoconductivity. Human osteoblasts were cultured on the surfaces of OCP and Mn-CHA thin films, and the cell attachment, proliferation and differentiation we evaluated up to 21 days. The cells showed a normal morphology and a very good rate of proliferation and viability in every experimental time. Alkaline phosphatase activity was always higher than the control and Ti groups. From days 7 to 21 collagen type I production was higher in comparison with control and Ti groups. The level of transforming growth factor beta 1 (TGF-beta1) was lower at 3 and 7 days, but reached the highest values during following experimental times (14 and 21 days). Our data demonstrate that both calcium phosphate coatings favour osteoblasts proliferation, activation of their metabolism and differentiation. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2381 / 2389

页数：9

共 48 条

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2-A

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