Image-based high-throughput quantification of cellular fat accumulation

被引：19

作者：

Dragunow, Mike ^{[1
]}

Cameron, Rachel

Narayan, Pritika

O'Carroll, Simon

机构：

[1] Univ Auckland, Fac Med & Hlth Sci, Dept Pharmacol, Auckland, New Zealand

[2] Univ Auckland, Fac Med & Hlth Sci, Dept Anat, Auckland, New Zealand

[3] Univ Auckland, Fac Med & Hlth Sci, Natl Res Ctr Growth & Dev, Auckland, New Zealand

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2007年 / 12卷 / 07期

关键词：

image analysis; lipids; high-content screening;

D O I：

10.1177/1087057107306502

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.

引用

页码：999 / 1005

页数：7

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