Quantitative trait loci for yellow pigment concentration and individual carotenoid compounds in durum wheat

被引:79
作者
Blanco, Antonio [1 ]
Colasuonno, Pasqualina [1 ]
Gadaleta, Agata [1 ]
Mangini, Giacomo [1 ]
Schiavulli, Adalgisa [1 ]
Simeone, Rosanna [1 ]
Digesu, Anna Maria [2 ]
De Vita, Pasquale [2 ]
Mastrangelo, Anna Maria [2 ]
Cattivelli, Luigi [2 ,3 ]
机构
[1] Univ Bari, Dept Environm & Agroforestry Biol & Chem, Sect Genet & Plant Breeding, I-70126 Bari, Italy
[2] CRA Cereal Res Ctr, I-71122 Foggia, Italy
[3] CRA Genom Res Ctr, I-29017 Fiorenzuola Darda, PC, Italy
关键词
Durum wheat; Pigment concentration; Carotenoid compounds; QTL mapping; PHYTOENE SYNTHASE; GENETIC-VARIABILITY; ENDOSPERM COLOR; QTL-ANALYSIS; ASSOCIATION; MICROSATELLITE; IDENTIFICATION; BIOSYNTHESIS; LIPOXYGENASE; ACCUMULATION;
D O I
10.1016/j.jcs.2011.07.002
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A defining factor for the commercial value of durum wheat pasta is its amber colour, which depends on the semolina yellow pigment concentration and on the oxidative enzymatic activity. Among carotenoids controlling yellow colour, the presence of beta-carotene is also important as precursors of vitamin A. The aim of the present study was to detect quantitative trait loci (QTL) for yellow pigment concentration, yellow index and individual carotenoid compounds (lutein, zeaxanthin, beta-cryptoxanthin, alpha-carotene and beta-carotene) in a durum segregant population. Total carotenoid concentration amounted to 37% of the yellow pigments, indicating unknown colour-producing compounds in the durum extracts. Lutein was the most abundant carotenoid, followed by zeaxanthin, alpha-carotene and beta-carotene, while beta-cryptoxanthin was a minor component. Phytoene synthase marker Psy-A1, 150 SSR and EST-SSR markers, and 345 DArT (R) markers, were used to construct the linkage map for subsequent QTL analysis. Clusters of QTL for total and/or one or more carotenoid compounds were detected on the same chromosome regions (2A, 3B, 5A and 7A) where QTL for yellow pigment concentration and yellow index were identified. The molecular markers associated to major QTL would be useful for marker-assisted selection programs to facilitate high carotenoid concentration with high nutritional carotenoid compounds in wheat grain. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:255 / 264
页数:10
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