SiRNAs do not induce RNA-dependent transcriptional silencing of retrovirus in human cells

被引：15

作者：

Haraguchi, Takeshi ^{[1
]}

Mizutani, Taketoshi ^{[1
]}

Yamamichi, Nobutake ^{[1
]}

Ito, Taiji ^{[1
]}

Minoguchi, Shigeru ^{[1
]}

Iba, Hideo ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Immunol & Microbiol, Div Host Parasite Interact,Minato Ku, Tokyo 108639, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 25期

基金：

日本学术振兴会;

关键词：

RNA interference; gene silencing; retrovirus; MLV; siRNA; shRNA;

D O I：

10.1016/j.febslet.2007.09.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-dependent transcriptional silencing (RdTS) has been reported to operate even in human cell lines. It is tempting to speculate that RdTS plays a role in retroviral gene silencing, considering that retroviral RNA transcripts harbor a U3 promoter sequence that is a potentially good source of doublestranded RNAs. To test this possibility, we constructed several model HeLaS3 cell lines expressing GFP driven by murine leukaemia virus (MLV)-long terminal repeat (LTR) and introduced a series of shRNAs that target the U3 region of the MLV-LTR. However, transcriptional gene silencing was not induced in most instances, in spite of the fact that processed shRNA was found in cellular nuclei, indicating that RdTS does not contribute to MLV gene silencing in host cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4949 / 4954

页数：6

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