Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting

被引：75

作者：

Hettick, Justin M. ^{[1
]}

Green, Brett J. ^{[1
]}

Buskirk, Amanda D. ^{[1
]}

Kashon, Michael L. ^{[1
]}

Slaven, James E. ^{[1
]}

Janotka, Erika ^{[1
]}

Blachere, Francoise M. ^{[1
]}

Schmechel, Detlef ^{[1
]}

Beezhold, Donald H. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, NIOSH, HELD, ACIB, Morgantown, WV 26505 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2008年 / 22卷 / 16期

关键词：

D O I：

10.1002/rcm.3649

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000-20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance. Published in 2008 by John Wiley & Sons, Ltd.

引用

页码：2555 / 2560

页数：6

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