Direct electron transfer between cytochrome c and a gold nanoparticles modified electrode

被引:192
作者
Wang, L [1 ]
Wang, EK [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jinlin, Peoples R China
基金
中国国家自然科学基金;
关键词
cytochrome c; gold nanoparticles; sol-gel; direct electron transfer;
D O I
10.1016/j.elecom.2003.10.004
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Quasi-reversible and direct electrochemistry of cytochrome c (cyt. c) has been obtained at a novel electrochemical interface constructed by self-assembling gold nanoparticles (GNPs) onto a three-dimensional silica gel network, without polishing or any modification of the surface. A cleaned gold electrode was first immersed in a hydrolyzed sol of the precursor (3-mercaptopropyl)-trimethoxysilane to assemble three-dimensional silica gel, then the GNPs were chemisorbed onto the thiol groups of the sol-gel network and modified the kinetic barrier of this self-assembled silicate film. Cyclic voltammetry and AC impendance spectroscopy were performed to evaluate electrochemical properties of the as prepared interface. These nanoparticle inhibits the adsorption of cyt. c onto bare electrode and acts as a bridge of electron transfer between protein and electrode. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:49 / 54
页数:6
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