Phylogenetic Analysis of Murine Leukemia Virus Sequences from Longitudinally Sampled Chronic Fatigue Syndrome Patients Suggests PCR Contamination Rather than Viral Evolution

被引：13

作者：

Katzourakis, Aris ^{[2
]}

Hue, Stephane ^{[1
]}

Kellam, Paul ^{[1
,3
]}

Towers, Greg J. ^{[1
]}

机构：

[1] UCL, MRC Ctr Med Mol Virol, Div Infect & Immun, London WC1E 6BT, England

[2] Univ Oxford, Dept Zool, Oxford OX1 3PS, England

[3] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 20期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

PROSTATE-CANCER; RETROVIRUS XMRV; UNITED-STATES; TREE SELECTION; BLOOD-DONORS; NO EVIDENCE; ABSENCE; MULTIPLE; CELLS; FAILURE;

D O I：

10.1128/JVI.00827-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Xenotropic murine leukemia virus (MLV)-related virus (XMRV) has been amplified from human prostate cancer and chronic fatigue syndrome (CFS) patient samples. Other studies failed to replicate these findings and suggested PCR contamination with a prostate cancer cell line, 22Rv1, as a likely source. MLV-like sequences have also been detected in CFS patients in longitudinal samples 15 years apart. Here, we tested whether sequence data from these samples are consistent with viral evolution. Our phylogenetic analyses strongly reject a model of within-patient evolution and demonstrate that the sequences from the first and second time points represent distinct endogenous murine retroviruses, suggesting contamination.

引用

页码：10909 / 10913

页数：5

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