AtPUB19, a U-Box E3 Ubiquitin Ligase, Negatively Regulates Abscisic Acid and Drought Responses in Arabidopsis thaliana

被引:117
作者
Liu, Yong-Chang [1 ]
Wu, Yao-Rong [2 ]
Huang, Xia-He [2 ]
Sun, Jie [1 ]
Xie, Qi [2 ]
机构
[1] Shihezi Univ, Coll Agr, Key Lab Oasis Ecoagr, Shihezi 832003, Xinjiang, Peoples R China
[2] Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
基金
中国国家自然科学基金;
关键词
U-box; ABA; drought stress; Arabidopsis; SIGNAL-TRANSDUCTION; CELL-DEATH; PROTEIN; STRESS; DOWNSTREAM; PROMOTES; SUBUNIT; ENCODES; PATHWAY; FAMILY;
D O I
10.1093/mp/ssr030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ubiquitination is an important protein post-translational modification, which is involved in various cellular processes in higher plants, and U-box E3 ligases play important roles in diverse functions in eukaryotes. Here, we describe the functions of Arabidopsis thaliana PUB19 (AtPUB19), which we demonstrated in an in vitro assay to encode a U-box type E3 ubiquitin ligase. AtPUB19 was up-regulated by drought, salt, cold, and abscisic acid (ABA). Down-regulation of AtPUB19 led to hypersensitivity to ABA, enhanced ABA-induced stomatal closing, and enhanced drought tolerance, while AtPUB19 overexpression resulted in the reverse phenotypes. Molecular analysis showed that the expression levels of a number of ABA and stress marker genes were altered in both AtPUB19 overexpressing and atpub19-1 mutant plants. In summary, our data show that AtPUB19 negatively regulates ABA and drought responses in A. thaliana.
引用
收藏
页码:938 / 946
页数:9
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