Variants of parvovirus B19: Bioinformatical evaluation of nested PCR assays

被引：8

作者：

Bergallo, Massimiliano ^{[1
]}

Costa, Cristina ^{[1
]}

Sidoti, Francesca ^{[1
]}

Novelli, Mauro ^{[2
]}

Ponti, Renata ^{[2
]}

Castagnoli, Carlotta ^{[3
,4
]}

Merlino, Chiara ^{[1
]}

Grazia Bernengo, Maria ^{[2
]}

Cavallo, Rossana ^{[1
]}

机构：

[1] Univ Turin, Dept Microbiol & Publ Hlth, Virol Unit, IT-10132 Turin, Italy

[2] Univ Turin, Dept Biomed Sci & Human Oncol, Dermatol Sect, IT-10132 Turin, Italy

[3] CTO Hosp, Dept Plast Surg, Turin, Italy

[4] CTO Hosp, Burn Unit Skin Bank, Turin, Italy

来源：

INTERVIROLOGY | 2008年 / 51卷 / 02期

关键词：

parvovirus B19; K71; V9; DNA detection;

D O I：

10.1159/000127429

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin. Copyright (C) 2008 S. Karger AG, Basel.

引用

页码：75 / 80

页数：6

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