A novel crossed microfluidic device for the precise positioning of proteins and vesicles

被引：28

作者：

Dusseiller, MR ^{[1
]}

Niederberger, B ^{[1
]}

Städler, B ^{[1
]}

Falconnet, D ^{[1
]}

Textor, M ^{[1
]}

Vörös, J ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Dept Mat, Surface Sci & Technol Lab, Biointerface Grp, CH-8093 Zurich, Switzerland

来源：

LAB ON A CHIP | 2005年 / 5卷 / 12期

关键词：

D O I：

10.1039/b509957a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein we present a novel way to create arrays of different proteins or lipid vesicles using a crossed microfluidic device. The concept relies on the combination of I) a designated two-step surface chemistry, which allows activation for subsequent binding events, and II) crossing microfluidic channels for the local functionalization by separated laminar streams. Besides its simplicity and cost efficiency, this concept has the advantage that it keeps the proteins in a hydrated environment throughout the experiment. We have demonstrated the feasibility of such a device to create a chessboard pattern of different fluorescently labeled lipid vesicles, which offers the possibility to contain biomolecules, drugs or membrane proteins.

引用

页码：1387 / 1392

页数：6

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