HPLC and HPLC-MS analysis of urinary Nε-monomethyl-lysine

被引：3

作者：

Kalász, H

Benkö, A

Szücs, Z

Szilàgyi, A

Szarvas, T

Lengyel, J

机构：

[1] Semmelweis Univ, Dept Pharmacol & Pharmacotherapy, H-1089 Budapest, Hungary

[2] Res Inst Med Plants, H-2011 Budakalasz, Hungary

[3] Inst Isotopes Co Ltd, Budapest, Hungary

[4] Semmelweis Univ, Cent Isotope Lab, H-1089 Budapest, Hungary

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2005年 / 43卷 / 04期

关键词：

D O I：

10.1093/chromsci/43.4.165

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Administration of 14C-labelled L-deprenyl to rats results in the urinary elimination of a 14C-labelled compound. The 9-fluorenylmethoxycarbonyl chloride-reacted urine sample is fractionated by high-performance liquid chromatography (HPLC) on an octadecyl silica stationary phase. Nε-Monomethyl-lysine is identified in the fraction containing the majority of the radioactivity. Structural elucidation is carried out using HPLC-mass spectrometry in atmospheric pressure chemical ionization mode. Identification of the 14C-labelled fragment in Nε-monomethyl- lysine is an experimental proof that an N-methylated amino acid is generated by transmethylation from a well-known drug. This type of transmethylation may have basic importance in the positive side effects of certain drugs.

引用

页码：165 / 168

页数：4

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